{"id":641,"date":"2019-04-19T15:18:45","date_gmt":"2019-04-19T15:18:45","guid":{"rendered":"https:\/\/williamslab.wordpress.ncsu.edu\/?p=641"},"modified":"2019-04-19T15:18:45","modified_gmt":"2019-04-19T15:18:45","slug":"substrate-promiscuity-of-isopentenyl-phosphate-kinase","status":"publish","type":"post","link":"https:\/\/williamslab.wordpress.ncsu.edu\/?p=641","title":{"rendered":"Substrate promiscuity of isopentenyl phosphate kinase"},"content":{"rendered":"<section id=\"section-1-en\" class=\"article-section article-section__abstract\" lang=\"en\" data-lang=\"en\">\n<div class=\"article-section__content en main\">\n<p>Isoprenoids are a large class of natural products with wide\u2010ranging applications. Synthetic biology approaches to the manufacture of isoprenoids and their new\u2010to\u2010nature derivatives are limited due to the provision in Nature of just two hemiterpene building blocks for isoprenoid biosynthesis. To address this limitation, artificial chemo\u2010enzymatic pathways such as the <a href=\"https:\/\/www.nature.com\/articles\/s41929-019-0240-8\">alcohol\u2010dependent hemiterpene pathway<\/a> (ADH) serve to leverage consecutive kinases to convert exogenous alcohols to pyrophosphates that could be coupled to downstream isoprenoid biosynthesis. To be successful, each kinase in this pathway should be permissive of a broad range of substrates. For the first time, <a href=\"http:\/\/dx.doi.org\/10.1002\/cbic.201900135\">we have probed the promiscuity of the second enzyme in the ADH pathway, isopentenyl phosphate kinase<\/a> from Thermoplasma acidophilum, towards a broad range of acceptor monophosphates. Subsequently, we evaluate the suitability of this enzyme to provide non\u2010natural pyrophosphates and provide a critical first step in characterizing the rate limiting steps in the artificial ADH pathway.<\/p>\n<p>See the publication at <a href=\"http:\/\/dx.doi.org\/10.1002\/cbic.201900135\">ChemBioChem<\/a>!<\/p>\n<p><a href=\"https:\/\/williamslab.wordpress.ncsu.edu\/?attachment_id=642\" rel=\"attachment wp-att-642\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-medium wp-image-642\" src=\"https:\/\/williamslab.wordpress.ncsu.edu\/files\/2019\/04\/graphical-abstract-v2-600x99.png\" alt=\"\" width=\"600\" height=\"99\" srcset=\"https:\/\/williamslab.wordpress.ncsu.edu\/files\/2019\/04\/graphical-abstract-v2-600x99.png 600w, https:\/\/williamslab.wordpress.ncsu.edu\/files\/2019\/04\/graphical-abstract-v2-768x127.png 768w, https:\/\/williamslab.wordpress.ncsu.edu\/files\/2019\/04\/graphical-abstract-v2-1200x198.png 1200w, https:\/\/williamslab.wordpress.ncsu.edu\/files\/2019\/04\/graphical-abstract-v2-500x83.png 500w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<\/div>\n<\/section>\n<section class=\"article-section article-section__supporting\">\n<div class=\"accordion article-accordion\"><\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Isoprenoids are a large class of natural products with wide\u2010ranging applications. Synthetic biology approaches to the manufacture of isoprenoids and their new\u2010to\u2010nature derivatives are limited due to the provision in Nature of just two hemiterpene building blocks for isoprenoid biosynthesis. To address this limitation, artificial chemo\u2010enzymatic pathways such as the alcohol\u2010dependent hemiterpene pathway (ADH) serve&hellip;<\/p>\n","protected":false},"author":767,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"ncst_dynamicHeaderBlockName":"","ncst_dynamicHeaderData":"","ncst_content_audit_freq":"","ncst_content_audit_date":"","ncst_content_audit_display":false,"ncst_backToTopFlag":"","footnotes":""},"categories":[8],"tags":[],"class_list":["post-641","post","type-post","status-publish","format-standard","hentry","category-lab-news"],"displayCategory":null,"acf":{"ncst_posts_meta_modified_date":null},"_links":{"self":[{"href":"https:\/\/williamslab.wordpress.ncsu.edu\/index.php?rest_route=\/wp\/v2\/posts\/641","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/williamslab.wordpress.ncsu.edu\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/williamslab.wordpress.ncsu.edu\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/williamslab.wordpress.ncsu.edu\/index.php?rest_route=\/wp\/v2\/users\/767"}],"replies":[{"embeddable":true,"href":"https:\/\/williamslab.wordpress.ncsu.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=641"}],"version-history":[{"count":1,"href":"https:\/\/williamslab.wordpress.ncsu.edu\/index.php?rest_route=\/wp\/v2\/posts\/641\/revisions"}],"predecessor-version":[{"id":643,"href":"https:\/\/williamslab.wordpress.ncsu.edu\/index.php?rest_route=\/wp\/v2\/posts\/641\/revisions\/643"}],"wp:attachment":[{"href":"https:\/\/williamslab.wordpress.ncsu.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=641"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/williamslab.wordpress.ncsu.edu\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=641"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/williamslab.wordpress.ncsu.edu\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=641"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}